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vector carrying ace2 coding sequences  (Addgene inc)


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    Structured Review

    Addgene inc vector carrying ace2 coding sequences
    Vector Carrying Ace2 Coding Sequences, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cas9+coding+sequence/ACE2+g1+%2B+Cas9+(Plasmid+%23153011)/pm40133950-166-13-23
    Average 91 stars, based on 1 article reviews
    vector carrying ace2 coding sequences - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

    Sequencing:

    Article Title: Pivotal roles of Plasmodium falciparum lysophospholipid acyltransferase 1 in cell cycle progression and cytostome internalization.
    Article Snippet: .. To remove Cas9 coding sequence from pUF-Cas9-pre-sgRNA (Addgene plasmid #137845; http://n2t.net/ addgene:137845; RRID:Addgene_137845), the plasmid was linearized at the XhoI and SalI restriction sites. .. Synthetic DNA containing Pflplat1 coding sequence followed by sequences of linker and triple c-myc was obtained (Azenta Life Sciences).

    Article Title: Upgraded CRISPR/Cas9 Tools for Tissue-Specific Mutagenesis in Drosophila
    Article Snippet: Gal4 5’ and 3’ homology arms were PCR-amplified using pDEST-APIGH (Addgene # 112804) as the template. .. Cas9 coding sequence was PCR-amplified using pEDST-APIC-Cas9 (Addgene # 121657) as the template. ..

    Article Title: Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids.
    Article Snippet: .. Lentiviral constructs, virus preparation and generation of inducible rescue lines Full-length human cDNA for BAP1 andBAP1C91A catalytically deadmutant were PCR-amplified from the Addgene plasmids #81024 and #81025 and cloned using theNheI and BamHI sites into the doxycycline-inducible lentiviral vector pCW-Cas9 (Addgene plasmid #50661) after removal of the Cas9 coding sequence, to generate the pCW-BAP1 and pCW-BAP1C91A plasmids. ..

    Article Title: An efficient vector-based CRISPR/Cas9 system in an Oreochromis mossambicus cell line using endogenous promoters
    Article Snippet: The pSBP2APuro plasmid was digested with PvuII and XhoI, blunted with New England Biolabs Quick Blunting kit (cat# E1201S), and re-ligated to remove excess unnecessary DNA sequence. .. The Cas9 coding sequence was digested from the pCS2-nCas9n (Addgene # 47929) plasmid with SalI and AgeI enzymes and ligated into the pSBP2APuro plasmid digested with SalI and XmaI enzymes (AgeI and XmaI have complementary cohesive ends) to make the Cas9P2APuroSB plasmid. ..

    Article Title: ANGPLT3 Impacts Not Only Proteinuria But Also Hyperlipidemia in Nephrotic Syndrome
    Article Snippet: .. Production of Cas9 mRNA and sgRNA T7 promoter was added to Cas9 coding sequence by PCR ampli cation using PX330 vector (Addgene) and the primer Cas9 (F and R) (Table S1). .. T7-Cas9 PCR product was gel puri ed by QIAquick Gel Extraction Kit (Qiagen, USA) and used as the template (500 ng) for in vitro transcription (IVT) using mMESSAGEmMACHINE T7 ULTRA Transcription Kit (Thermo Fisher Scienti c, USA).

    Article Title: Pivotal roles of Plasmodium falciparum lysophospholipid acyltransferase 1 in cell cycle progression and cytostome internalization
    Article Snippet: .. To remove Cas9 coding sequence from pUF-Cas9-pre-sgRNA (Addgene plasmid #137845; http://n2t.net/addgene:137845 ; RRID:Addgene_137845), the plasmid was linearized at the XhoI and SalI restriction sites. .. Synthetic DNA containing Pflplat1 coding sequence followed by sequences of linker and triple c-myc was obtained (Azenta Life Sciences).

    Article Title: Ingestion of single guide RNAs induces gene overexpression and extends lifespan in Caenorhabditis elegans via CRISPR activation
    Article Snippet: .. C. elegans dCas9::VP64 overexpression vector: We designed a DNA fragment flanked by attB1 and attB2 recombination sites and containing a Cas9 coding sequence optimized for efficient expression in C. elegans (based on Addgene plasmid #47549) ( ). ..

    Plasmid Preparation:

    Article Title: Pivotal roles of Plasmodium falciparum lysophospholipid acyltransferase 1 in cell cycle progression and cytostome internalization.
    Article Snippet: .. To remove Cas9 coding sequence from pUF-Cas9-pre-sgRNA (Addgene plasmid #137845; http://n2t.net/ addgene:137845; RRID:Addgene_137845), the plasmid was linearized at the XhoI and SalI restriction sites. .. Synthetic DNA containing Pflplat1 coding sequence followed by sequences of linker and triple c-myc was obtained (Azenta Life Sciences).

    Article Title: Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids.
    Article Snippet: .. Lentiviral constructs, virus preparation and generation of inducible rescue lines Full-length human cDNA for BAP1 andBAP1C91A catalytically deadmutant were PCR-amplified from the Addgene plasmids #81024 and #81025 and cloned using theNheI and BamHI sites into the doxycycline-inducible lentiviral vector pCW-Cas9 (Addgene plasmid #50661) after removal of the Cas9 coding sequence, to generate the pCW-BAP1 and pCW-BAP1C91A plasmids. ..

    Article Title: An efficient vector-based CRISPR/Cas9 system in an Oreochromis mossambicus cell line using endogenous promoters
    Article Snippet: The pSBP2APuro plasmid was digested with PvuII and XhoI, blunted with New England Biolabs Quick Blunting kit (cat# E1201S), and re-ligated to remove excess unnecessary DNA sequence. .. The Cas9 coding sequence was digested from the pCS2-nCas9n (Addgene # 47929) plasmid with SalI and AgeI enzymes and ligated into the pSBP2APuro plasmid digested with SalI and XmaI enzymes (AgeI and XmaI have complementary cohesive ends) to make the Cas9P2APuroSB plasmid. ..

    Article Title: ANGPLT3 Impacts Not Only Proteinuria But Also Hyperlipidemia in Nephrotic Syndrome
    Article Snippet: .. Production of Cas9 mRNA and sgRNA T7 promoter was added to Cas9 coding sequence by PCR ampli cation using PX330 vector (Addgene) and the primer Cas9 (F and R) (Table S1). .. T7-Cas9 PCR product was gel puri ed by QIAquick Gel Extraction Kit (Qiagen, USA) and used as the template (500 ng) for in vitro transcription (IVT) using mMESSAGEmMACHINE T7 ULTRA Transcription Kit (Thermo Fisher Scienti c, USA).

    Article Title: Pivotal roles of Plasmodium falciparum lysophospholipid acyltransferase 1 in cell cycle progression and cytostome internalization
    Article Snippet: .. To remove Cas9 coding sequence from pUF-Cas9-pre-sgRNA (Addgene plasmid #137845; http://n2t.net/addgene:137845 ; RRID:Addgene_137845), the plasmid was linearized at the XhoI and SalI restriction sites. .. Synthetic DNA containing Pflplat1 coding sequence followed by sequences of linker and triple c-myc was obtained (Azenta Life Sciences).

    Article Title: Ingestion of single guide RNAs induces gene overexpression and extends lifespan in Caenorhabditis elegans via CRISPR activation
    Article Snippet: .. C. elegans dCas9::VP64 overexpression vector: We designed a DNA fragment flanked by attB1 and attB2 recombination sites and containing a Cas9 coding sequence optimized for efficient expression in C. elegans (based on Addgene plasmid #47549) ( ). ..

    Polymerase Chain Reaction:

    Article Title: Upgraded CRISPR/Cas9 Tools for Tissue-Specific Mutagenesis in Drosophila
    Article Snippet: Gal4 5’ and 3’ homology arms were PCR-amplified using pDEST-APIGH (Addgene # 112804) as the template. .. Cas9 coding sequence was PCR-amplified using pEDST-APIC-Cas9 (Addgene # 121657) as the template. ..

    Article Title: Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids.
    Article Snippet: .. Lentiviral constructs, virus preparation and generation of inducible rescue lines Full-length human cDNA for BAP1 andBAP1C91A catalytically deadmutant were PCR-amplified from the Addgene plasmids #81024 and #81025 and cloned using theNheI and BamHI sites into the doxycycline-inducible lentiviral vector pCW-Cas9 (Addgene plasmid #50661) after removal of the Cas9 coding sequence, to generate the pCW-BAP1 and pCW-BAP1C91A plasmids. ..

    Article Title: ANGPLT3 Impacts Not Only Proteinuria But Also Hyperlipidemia in Nephrotic Syndrome
    Article Snippet: .. Production of Cas9 mRNA and sgRNA T7 promoter was added to Cas9 coding sequence by PCR ampli cation using PX330 vector (Addgene) and the primer Cas9 (F and R) (Table S1). .. T7-Cas9 PCR product was gel puri ed by QIAquick Gel Extraction Kit (Qiagen, USA) and used as the template (500 ng) for in vitro transcription (IVT) using mMESSAGEmMACHINE T7 ULTRA Transcription Kit (Thermo Fisher Scienti c, USA).

    Construct:

    Article Title: Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids.
    Article Snippet: .. Lentiviral constructs, virus preparation and generation of inducible rescue lines Full-length human cDNA for BAP1 andBAP1C91A catalytically deadmutant were PCR-amplified from the Addgene plasmids #81024 and #81025 and cloned using theNheI and BamHI sites into the doxycycline-inducible lentiviral vector pCW-Cas9 (Addgene plasmid #50661) after removal of the Cas9 coding sequence, to generate the pCW-BAP1 and pCW-BAP1C91A plasmids. ..

    Virus:

    Article Title: Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids.
    Article Snippet: .. Lentiviral constructs, virus preparation and generation of inducible rescue lines Full-length human cDNA for BAP1 andBAP1C91A catalytically deadmutant were PCR-amplified from the Addgene plasmids #81024 and #81025 and cloned using theNheI and BamHI sites into the doxycycline-inducible lentiviral vector pCW-Cas9 (Addgene plasmid #50661) after removal of the Cas9 coding sequence, to generate the pCW-BAP1 and pCW-BAP1C91A plasmids. ..

    Clone Assay:

    Article Title: Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids.
    Article Snippet: .. Lentiviral constructs, virus preparation and generation of inducible rescue lines Full-length human cDNA for BAP1 andBAP1C91A catalytically deadmutant were PCR-amplified from the Addgene plasmids #81024 and #81025 and cloned using theNheI and BamHI sites into the doxycycline-inducible lentiviral vector pCW-Cas9 (Addgene plasmid #50661) after removal of the Cas9 coding sequence, to generate the pCW-BAP1 and pCW-BAP1C91A plasmids. ..

    other:

    Article Title: Compositions and methods of engineered CRISPR-Cas9 systems using split-nexus Cas9-associated polynucleotides
    Article Snippet: Production of Cas9 mRNA and sn1-casRNA/sn2-casRNA A T7 promoter is added to Cas9 coding region optimized for mammalian expression (e.g., the Cas9 coding sequence can be PCR amplified from pX330-U6-Chimeric BB-CBh-hSpCas9; Addgene, Cambridge, Mass.).

    Over Expression:

    Article Title: Ingestion of single guide RNAs induces gene overexpression and extends lifespan in Caenorhabditis elegans via CRISPR activation
    Article Snippet: .. C. elegans dCas9::VP64 overexpression vector: We designed a DNA fragment flanked by attB1 and attB2 recombination sites and containing a Cas9 coding sequence optimized for efficient expression in C. elegans (based on Addgene plasmid #47549) ( ). ..

    Expressing:

    Article Title: Ingestion of single guide RNAs induces gene overexpression and extends lifespan in Caenorhabditis elegans via CRISPR activation
    Article Snippet: .. C. elegans dCas9::VP64 overexpression vector: We designed a DNA fragment flanked by attB1 and attB2 recombination sites and containing a Cas9 coding sequence optimized for efficient expression in C. elegans (based on Addgene plasmid #47549) ( ). ..



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    Image Search Results


    Fig. 2 The modified 3TC scaffold boosts SpCas9 gRNA expression levels compared to the original 4T scaffold. (A) DNA sequence of the 4T and modified 3TC scaffolds. (B) Relative quantification (RQ) of mDmd gRNA delivered by nucleofection of PX459.V2 (4T) to C2C12 cells, by qRT-PCR. Mean Log2RQ ± 95% CI; n = 3. One-way ANOVA with Dunnett’s multiple comparisons test was performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001. (C, D, E) Comparison of the relative quantities of mDmd Sp gRNA, delivered by PX459.V2, pdg459.V2 (2 × 4T) and PX459.V3 (3TC), measured by qRT-PCR. Mean Log2RQ ± 95% CI; n = 3. One-way ANOVA with Tukey’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001

    Journal: BMC genomics

    Article Title: Optimal SpCas9- and SaCas9-mediated gene editing by enhancing gRNA transcript levels through scaffold poly-T tract reduction.

    doi: 10.1186/s12864-025-11317-2

    Figure Lengend Snippet: Fig. 2 The modified 3TC scaffold boosts SpCas9 gRNA expression levels compared to the original 4T scaffold. (A) DNA sequence of the 4T and modified 3TC scaffolds. (B) Relative quantification (RQ) of mDmd gRNA delivered by nucleofection of PX459.V2 (4T) to C2C12 cells, by qRT-PCR. Mean Log2RQ ± 95% CI; n = 3. One-way ANOVA with Dunnett’s multiple comparisons test was performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001. (C, D, E) Comparison of the relative quantities of mDmd Sp gRNA, delivered by PX459.V2, pdg459.V2 (2 × 4T) and PX459.V3 (3TC), measured by qRT-PCR. Mean Log2RQ ± 95% CI; n = 3. One-way ANOVA with Tukey’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001

    Article Snippet: SaCas9Puro.V2 was generated by replacing the SpCas9 coding sequences of PX459.V2 with SaCas9 coding sequences from PX601 (Addgene #61591).

    Techniques: Modification, Expressing, Sequencing, Quantitative Proteomics, Quantitative RT-PCR, Comparison

    Fig. 5 Editing efficiencies of high-fidelity SpCas9s with the 3TC scaffold. Comparison of PX459.V2 SpCas9-HF1 (4T), PX459.V3 SpCas9-HF1 (3TC), PX459.V2 eSpCas9(1.1) (4T) and PX459.V3 eSpCas9(1.1) (3TC) plasmids de livered by lipofection at a (A) high and (B) low plasmid dose without puro mycin selection in HEK239T cells, assessed by deep amplicon sequencing. Mean ± SEM; n = 3. Two-way ANOVA with Šídák’s multiple comparisons test; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. (C) Editing efficiencies of hDMD-B in the G19 gRNA configuration with WT and high-fidelity Sp-Cas9 plasmids delivered by nucleofection with puromycin selection in HEK293Ts. Two- way ANOVA with Šídák’s multiple comparisons test; *p ≤ 0.05, ***p ≤ 0.001

    Journal: BMC genomics

    Article Title: Optimal SpCas9- and SaCas9-mediated gene editing by enhancing gRNA transcript levels through scaffold poly-T tract reduction.

    doi: 10.1186/s12864-025-11317-2

    Figure Lengend Snippet: Fig. 5 Editing efficiencies of high-fidelity SpCas9s with the 3TC scaffold. Comparison of PX459.V2 SpCas9-HF1 (4T), PX459.V3 SpCas9-HF1 (3TC), PX459.V2 eSpCas9(1.1) (4T) and PX459.V3 eSpCas9(1.1) (3TC) plasmids de livered by lipofection at a (A) high and (B) low plasmid dose without puro mycin selection in HEK239T cells, assessed by deep amplicon sequencing. Mean ± SEM; n = 3. Two-way ANOVA with Šídák’s multiple comparisons test; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. (C) Editing efficiencies of hDMD-B in the G19 gRNA configuration with WT and high-fidelity Sp-Cas9 plasmids delivered by nucleofection with puromycin selection in HEK293Ts. Two- way ANOVA with Šídák’s multiple comparisons test; *p ≤ 0.05, ***p ≤ 0.001

    Article Snippet: SaCas9Puro.V2 was generated by replacing the SpCas9 coding sequences of PX459.V2 with SaCas9 coding sequences from PX601 (Addgene #61591).

    Techniques: Comparison, Plasmid Preparation, Selection, Amplification, Sequencing